Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Unbiased optical mapping of telomere-integrated endogenous human herpesvirus 6

doi: 10.1073/pnas.2011872117

Figure Lengend Snippet: Specifications and coverage of the data collected from the Bionano Saphyr system

Article Snippet: Targeting at least 480 Gbp of data per sample, high-throughput imaging was performed using Bionano Genomics second-generation Saphyr Systems and chips with Instrument Control Software version 4.7.18339.1.

Techniques:

Journal: Poultry Science

Article Title: High-throughput Oxford Nanopore sequencing-based approach for the multilocus sequence typing analysis of large-scale avian Escherichia coli study in Mississippi

doi: 10.1016/j.psj.2024.104067

Figure Lengend Snippet: Workflow of multilocus sequence typing (MLST) using Oxford Nanopore Technologies (ONT). A general workflow for the DNA extraction, amplicon generation, amplicon barcoding, ONT sequencing, and data analysis.

Article Snippet: As the era moves to third-generation sequencing, the Oxford Nanopore Technologies ( ONT ) system emerges as a promising alternative to both Sanger and second-generation sequencing approaches, especially for high-throughput, long-read sequencing studies ( ).

Techniques: Sequencing, DNA Extraction, Amplification

Journal: Poultry Science

Article Title: High-throughput Oxford Nanopore sequencing-based approach for the multilocus sequence typing analysis of large-scale avian Escherichia coli study in Mississippi

doi: 10.1016/j.psj.2024.104067

Figure Lengend Snippet: Summary of the ONT sequencing results from ONT-MLST Beta workflow.

Article Snippet: As the era moves to third-generation sequencing, the Oxford Nanopore Technologies ( ONT ) system emerges as a promising alternative to both Sanger and second-generation sequencing approaches, especially for high-throughput, long-read sequencing studies ( ).

Techniques: Sequencing, Generated

Journal: Poultry Science

Article Title: High-throughput Oxford Nanopore sequencing-based approach for the multilocus sequence typing analysis of large-scale avian Escherichia coli study in Mississippi

doi: 10.1016/j.psj.2024.104067

Figure Lengend Snippet: Simulation of accuracy of the allele and ST calls for each subsample of the ONT compared to the SRST2 (Illumina) output. The sequencing data of 66 E. coli isolates from ONT-MLST Beta workflow and Illumina platform was used in this in silico simulation analysis.

Article Snippet: As the era moves to third-generation sequencing, the Oxford Nanopore Technologies ( ONT ) system emerges as a promising alternative to both Sanger and second-generation sequencing approaches, especially for high-throughput, long-read sequencing studies ( ).

Techniques: Sequencing, In Silico

Journal: The EPMA Journal

Article Title: The crucial role of multiomic approach in cancer research and clinically relevant outcomes

doi: 10.1007/s13167-018-0128-8

Figure Lengend Snippet: Parameters of partial platforms

Article Snippet: The third generation of sequencing technology such as PacBio RS and Oxford Nanopore sequencing is developed to solve the shortcomings of the second-generation [ ], with fundamental feature of the single molecule sequencing but not requirement of any PCR process, which effectively avoids the PCR bias caused by the system error, improve the read length, and maintain the advantages of high-throughput and low cost of the second-generation technology.

Techniques: Sequencing, Ligation